Jpet #72553 1 Potentiation of the Osmosensitive Release of Taurine and D- Aspartate from Sh-sy5y Neuroblastoma Cells following Activation of M3 Muscarinic Cholinergic Receptors
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چکیده
216 words Introduction: 750 words Discussion: 1391 words Non-standard abbreviations: mAChR, muscarinic cholinergic receptor; DIDS, 4,4’ diisothiocyanatostilbene-2,2’-disulfonic acid; NPPB, 5-nitro-2-(3-phenylpropylamino) benzoic acid; Oxo-M, oxotremorine-M; DMEM, Dulbecco’s modified Eagle’s medium; 4DAMP, 4-diphenylacetoxy-N-methylpiperidine methiodide; AF-DX 116, 11-[[2[(diethylamino)methyl]-1-piperidinyl]acetyl]-5,11-dihydro-6H-pyrido[2,3b][1,4]benzodiazepin-6-one; SITS, 4-acetamido-4’-isothiocyanatostilbene 2,2’disulfonic acid; VSOAC, volume-sensitive organic anion channel; mpV, monoperoxo vanadium (picolinato) Section assignment: Neuropharmacology This article has not been copyedited and formatted. The final version may differ from this version. JPET Fast Forward. Published on August 3, 2004 as DOI: 10.1124/jpet.104.072553 at A PE T Jornals on O cber 9, 2016 jpet.asjournals.org D ow nladed from
منابع مشابه
Activation of muscarinic cholinergic receptors on human SH-SY5Y neuroblastoma cells enhances both the influx and efflux of K+ under conditions of hypo-osmolarity.
The ability of receptor activation to regulate osmosensitive K+ fluxes (monitored as 86Rb+) in SH-SY5Y neuroblastoma has been examined. Incubation of SH-SY5Y cells in buffers rendered increasingly hypotonic by a reduction in NaCl concentration resulted in an enhanced basal efflux of Rb+ (threshold of release, 200 mOsM) but had no effect on Rb(+) influx. Addition of the muscarinic cholinergic ag...
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